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Warning: call dbDisconnect() when finished working with a connection #15

@dariomel

Description

@dariomel

this warning comes out in a situation that is specific to my application and is too complicated to describe and reproduce here.
However, the warning also appears when the following commands are executed immediately after starting R:

options(warn=1)
library(celldex)
Loading required package: SummarizedExperiment
Loading required package: MatrixGenerics
Loading required package: matrixStats

Attaching package: ‘MatrixGenerics’

The following objects are masked from ‘package:matrixStats’:

    colAlls, colAnyNAs, colAnys, colAvgsPerRowSet, colCollapse,
    colCounts, colCummaxs, colCummins, colCumprods, colCumsums,
    colDiffs, colIQRDiffs, colIQRs, colLogSumExps, colMadDiffs,
    colMads, colMaxs, colMeans2, colMedians, colMins, colOrderStats,
    colProds, colQuantiles, colRanges, colRanks, colSdDiffs, colSds,
    colSums2, colTabulates, colVarDiffs, colVars, colWeightedMads,
    colWeightedMeans, colWeightedMedians, colWeightedSds,
    colWeightedVars, rowAlls, rowAnyNAs, rowAnys, rowAvgsPerColSet,
    rowCollapse, rowCounts, rowCummaxs, rowCummins, rowCumprods,
    rowCumsums, rowDiffs, rowIQRDiffs, rowIQRs, rowLogSumExps,
    rowMadDiffs, rowMads, rowMaxs, rowMeans2, rowMedians, rowMins,
    rowOrderStats, rowProds, rowQuantiles, rowRanges, rowRanks,
    rowSdDiffs, rowSds, rowSums2, rowTabulates, rowVarDiffs, rowVars,
    rowWeightedMads, rowWeightedMeans, rowWeightedMedians,
    rowWeightedSds, rowWeightedVars

Loading required package: GenomicRanges
Loading required package: stats4
Loading required package: BiocGenerics

Attaching package: ‘BiocGenerics’

The following objects are masked from ‘package:stats’:

    IQR, mad, sd, var, xtabs

The following objects are masked from ‘package:base’:

    anyDuplicated, append, as.data.frame, basename, cbind, colnames,
    dirname, do.call, duplicated, eval, evalq, Filter, Find, get, grep,
    grepl, intersect, is.unsorted, lapply, Map, mapply, match, mget,
    order, paste, pmax, pmax.int, pmin, pmin.int, Position, rank,
    rbind, Reduce, rownames, sapply, setdiff, sort, table, tapply,
    union, unique, unsplit, which.max, which.min

Loading required package: S4Vectors

Attaching package: ‘S4Vectors’

The following objects are masked from ‘package:base’:

    expand.grid, I, unname

Loading required package: IRanges
Loading required package: GenomeInfoDb
Loading required package: Biobase
Welcome to Bioconductor

    Vignettes contain introductory material; view with
    'browseVignettes()'. To cite Bioconductor, see
    'citation("Biobase")', and for packages 'citation("pkgname")'.


Attaching package: ‘Biobase’

The following object is masked from ‘package:MatrixGenerics’:

    rowMedians

The following objects are masked from ‘package:matrixStats’:

    anyMissing, rowMedians
NovershternHematopoieticData(ensembl=T)
snapshotDate(): 2021-10-19
see ?celldex and browseVignettes('celldex') for documentation
loading from cache
see ?celldex and browseVignettes('celldex') for documentation
loading from cache
snapshotDate(): 2021-10-20
loading from cache
require(“ensembldb”)
Warning: Unable to map 3426 of 13276 requested IDs.
class: SummarizedExperiment 
dim: 9850 211 
metadata(0):
assays(1): logcounts
rownames(9850): ENSG00000175899 ENSG00000128274 ... ENSG00000159840
  ENSG00000074755
rowData names(0):
colnames(211): GSM609632 GSM609633 ... GSM609841 GSM609842
colData names(3): label.main label.fine label.ont
gc()
Warning: call dbDisconnect() when finished working with a connection
           used  (Mb) gc trigger  (Mb) max used  (Mb)
Ncells  8843461 472.3   15254835 814.7 13519245 722.1
Vcells 17372432 132.6   27509457 209.9 22857881 174.4
sessionInfo()
R version 4.1.1 (2021-08-10)
Platform: x86_64-pc-linux-gnu (64-bit)
Running under: Ubuntu 16.04.7 LTS

Matrix products: default
BLAS:   /mnt/share/apps/tools/R/R-4.1.1/lib/R/lib/libRblas.so
LAPACK: /mnt/share/apps/tools/R/R-4.1.1/lib/R/lib/libRlapack.so

locale:
 [1] LC_CTYPE=en_US.UTF-8       LC_NUMERIC=C              
 [3] LC_TIME=en_US.UTF-8        LC_COLLATE=en_US.UTF-8    
 [5] LC_MONETARY=en_US.UTF-8    LC_MESSAGES=en_US.UTF-8   
 [7] LC_PAPER=en_US.UTF-8       LC_NAME=C                 
 [9] LC_ADDRESS=C               LC_TELEPHONE=C            
[11] LC_MEASUREMENT=en_US.UTF-8 LC_IDENTIFICATION=C       

attached base packages:
[1] stats4    stats     graphics  grDevices utils     datasets  methods  
[8] base     

other attached packages:
 [1] ensembldb_2.18.2            AnnotationFilter_1.18.0    
 [3] GenomicFeatures_1.46.1      AnnotationDbi_1.56.2       
 [5] celldex_1.4.0               SummarizedExperiment_1.24.0
 [7] Biobase_2.54.0              GenomicRanges_1.46.1       
 [9] GenomeInfoDb_1.30.0         IRanges_2.28.0             
[11] S4Vectors_0.32.3            BiocGenerics_0.40.0        
[13] MatrixGenerics_1.6.0        matrixStats_0.61.0         

loaded via a namespace (and not attached):
 [1] httr_1.4.2                    bit64_4.0.5                  
 [3] AnnotationHub_3.2.0           DelayedMatrixStats_1.16.0    
 [5] shiny_1.7.1                   assertthat_0.2.1             
 [7] interactiveDisplayBase_1.32.0 BiocManager_1.30.16          
 [9] BiocFileCache_2.2.0           blob_1.2.2                   
[11] Rsamtools_2.10.0              GenomeInfoDbData_1.2.7       
[13] yaml_2.2.1                    progress_1.2.2               
[15] BiocVersion_3.14.0            pillar_1.6.4                 
[17] RSQLite_2.2.8                 lattice_0.20-45              
[19] glue_1.5.1                    digest_0.6.28                
[21] promises_1.2.0.1              XVector_0.34.0               
[23] htmltools_0.5.2               httpuv_1.6.3                 
[25] Matrix_1.3-4                  XML_3.99-0.8                 
[27] pkgconfig_2.0.3               biomaRt_2.50.1               
[29] zlibbioc_1.40.0               purrr_0.3.4                  
[31] xtable_1.8-4                  later_1.3.0                  
[33] BiocParallel_1.28.1           tibble_3.1.6                 
[35] KEGGREST_1.34.0               generics_0.1.1               
[37] ellipsis_0.3.2                cachem_1.0.6                 
[39] withr_2.4.3                   lazyeval_0.2.2               
[41] magrittr_2.0.1                crayon_1.4.2                 
[43] mime_0.12                     memoise_2.0.0                
[45] fansi_0.5.0                   xml2_1.3.2                   
[47] tools_4.1.1                   prettyunits_1.1.1            
[49] hms_1.1.1                     BiocIO_1.4.0                 
[51] lifecycle_1.0.1               stringr_1.4.0                
[53] DelayedArray_0.20.0           Biostrings_2.62.0            
[55] compiler_4.1.1                rlang_0.4.12                 
[57] grid_4.1.1                    RCurl_1.98-1.5               
[59] rjson_0.2.20                  rappdirs_0.3.3               
[61] bitops_1.0-7                  ExperimentHub_2.2.0          
[63] restfulr_0.0.13               DBI_1.1.1                    
[65] curl_4.3.2                    R6_2.5.1                     
[67] GenomicAlignments_1.30.0      rtracklayer_1.54.0           
[69] dplyr_1.0.7                   fastmap_1.1.0                
[71] bit_4.0.4                     utf8_1.2.2                   
[73] filelock_1.0.2                ProtGenerics_1.26.0          
[75] stringi_1.7.5                 parallel_4.1.1               
[77] Rcpp_1.0.7                    vctrs_0.3.8                  
[79] png_0.1-7                     dbplyr_2.1.1                 
[81] tidyselect_1.1.1              sparseMatrixStats_1.6.0      

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